Cytologic examination of ascitic fluid in a patient with pleuralbased. However, physicians performing paracentesis in these patients, often send fluid to the cytology laboratory, sometimes specifically looking for hepatocellular carcinoma hcc. Top tips for ascitic tap paracentesis always send the most fluid to cytology, especially if malignancy is suspected. Pleural fluid or ascites cytology specimen preparation. Ppt ascites powerpoint presentation free to download. Analysis of ascitic fluid for cytological and biochemical findings. Patients with cirrhosis develop ascites for physiologic reasons that are unrelated to malignant progression. Analysis of ascetic fluid for cytological and biochemical. This may result from either a traumatic tap or malignancy. It may also be called an abdominal tap or paracentesis. The more fluid sent, the higher the diagnostic yield. Book an appointment at a high quality center in hyderabad. Fluid analysis cytology was positive for adenocarcinoma. It is recommended that the initial assessment should concentrate on history, examination, and basic tests on ascitic fluid to assess the serumascites albumin gradient.
Only one case of primary peritoneal mesothelioma was detected on asctic fluid cytology. Pseudomyxoma peritonei pmp is a rare condition characterized by gelatinous ascites. Ascitic fluid glucose, ldh and total proteins may be helpful in ddx. A simplified description of ascitic fluid analysis. Isolation and characterization of tumor cells from the. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. Fluid was obtained by aspiration of ascitic fluid 74 patients or by peritoneal washings at the time oflaparotomy 20 patients. A peritoneal fluid culture is a test that is performed on a small sample of peritoneal fluid. Cytology and fluid analysis of the acute abdomen tesis can be helpful in making a definitive diagnosis more rap idly. Diagnostic utility of cell block method versus cytospin. Primary diagnosis of lymphomas from ascitic fluid is rare. Marilia arcadipane, luis alberto lage and juliana pereira.
This monograph essentials of fluid cytology is written for practicing. Free cytology books download ebooks online textbooks. Label the container with patients name, hospital number, room number, date, and type of specimen. Abdominal paracentesis is a bedside procedure where the ascitic fluid is collected by insertion of needle into the peritoneal cavity. Ascitic fluid cytomorphologic useless findings cytoplasmic vacuoles signet ring cells individual psammoma bodies cell within cell. A relatively simple bedside procedure in which one inserts a needle into the abdomen, thereby evacuating either a small amount of ascites fluid for diagnostic purposes, or large amounts of fluid for therapeutic purposes. Ascitic fluid may be used to help determine the etiology of ascites, as well as to evaluate for infection or presence of cancer. The authors have provided a general picture of the field, the details of which may be filled in by reference to the many fine sources available. A male lifelong nonsmoker aged 58 years with no prior asbestos exposure complained of gradual worsening breathlessness over 3 months.
Download scientific diagram cytology of ascitic fluid and prostate acid. Ascites is abnormal excessive accumulation of fluid in peritoneal cavity it is the most common of the 3 major complications of cirrhosis ascites, hepatic encephalopathy and bleeding varices hepatology 1987. Diagnostic algorithm for determining primary tumor sites using. Ppt cytology powerpoint presentation free to view id. We decided to proceed with transplantation as there was no sign of active bacterial peritonitis. Another cytology technique is to gently scrape or brush some cells from the organ or tissue being tested. Analysis of ascetic fluid for cytological and biochemical findingsx. Get 1550% off on diagnostic tests at top diagnostic centers. Ascitic fluid ascites is the abnormal accumulation of serous fluid in the peritoneal abdominal cavity and the fluid thus accumulated is referred to as ascetic fluid. Mean age of presentation in non malignant cases was 46. Ascitic fluid for cytology prices starting at rs 600 in.
If you want to gain a larger sample use a 50ml syringe to aspirate fluid. Find powerpoint presentations and slides using the power of, find free presentations research about body fluid cytology ppt. In five of the false negative cases the initial investigation after presentation was an ascitic fluid aspiration. How valuable is ascitic cytology in the detection and management of. Cytology in peritoneal carcinomatosis sensitivity increased by centrifuging large. Ascitic fluid analysis in hepatocellular carcinoma. Cytokeratins 7 and 20 are differentially expressed by different normal tissues. Peritoneal paracentesis adult, peds uc san diego health. Nonspecific finding in women in ascitic fluids and pelvic washings. Ascitic fluid cytology numerous pleomorphic lymphoid. Pleural effusion showing monomorphic population of atypical cells in a case of. Fluid analysis fluid analysis begins with appropriate sample collection. Ascitic fluid what do you do muhammad asim rana mbbs, mrcp, mrcps, fccp, edic, sfccm critical care medicine king saud medical city 2. The fresh fluid was sent to the cytology laboratory where filter preparations, smears, and cell blocks were prepared by standard methods.
Patient was discharged on synthroid 112 mcg daily, protonix 40. Effusion cytology is the study of individual cells. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. We have investigated the diagnostic yield of these specimens. Work up laboratory studies in patients with newonset ascites of unknown origin, peritoneal fluid should be sent for. Shidham table of contents factors leading to potential diagnostic pitfalls 43 surfacetensionrelated alterations in cytomorphology 43 improper specimen. A minimum of 10,000 red blood cellsl is required for ascitic fluid to appear pink. Cytology of ascitic fluid and prostate acid phosphatase pap test. Under ultrasound guidance the patient underwent paracentesis for removal of 3,500 ml of thin, nonpurulent, ascitic fluid. The article deals with cytopathology specimens from spaces lined with mesothelium, i.
To determine the presence of infection in the ascitic fluid c. Exfoliative cytology female genital tract fgt the cytological specimens collected from fgt include cervical. Pdf cytomorphological assessment of different body fluids. Cytologic examination of ascitic fluid in a patient with pleuralbased mass. Vijay kumar bodal, preeti banasal, manjit singh bal, anil kumar suri, ranjeev bhagat, navneet kaur, mohanveer kaur and anikita goel. The bestknown cytology test that samples cells this way is the pap test. This book is concerned with a discussion of the basic facts, concepts, and problems of cellular biology. Tumors of borderline malignancy were included in the series. Too much hope is placed on ascitic cytology to provide the diagnosis at the expense of other investigations. Peritoneal fluid cytology in patients with ovarian cancer. Ascitic fluids exist to lubricate the organs and ensure. Variation in the microscopic impression obtained by cytospin smears and cell block methods in.
Presentation will help the medical residents diagnose the cause of fluid accumulation in abdomen and thus will. Tumor cells in ascites are a major source of disease recurrence in ovarian cancer patients. This video provides instruction on the proper way to aliquot and transport pleural fluid or ascites cytology specimens to a pathology. View and download powerpoint presentations on body fluid cytology ppt. Application of cytokeratin 7 and 20 immunohistochemistry. Mesothelial cytopathology is a large part of cytopathology. Analysis of ascitic fluid for cytological and biochemical. Peritoneal tuberculosis as a cause of ascites in a patient. Aim of the presentation is to give a very clear understanding about the analysis of ascities. Cytology showed benign mesothelial cells and mixed inflammatory cells. Normally contains very little fluid effusion develops from imbalance of fluid formation and removal. Ascitic fluid is a fluid that is also called peritoneal cavity fluid. Advanced health practitioner must perform this procedure at least three times per year.
Although the histologic attributes of pmp have been well studied, the cytologic features remain. Winner of the standing ovation award for best powerpoint templates from presentations magazine. Department of pathology, government medical college, patila, punjab, india. How valuable is ascitic cytology in the detection and. Abnormal results are an indication for further nonroutine tests on another specimen. As regards cytological examination for malignant cells in ascitic fluid, positive cytology was recorded. Each candidate will be initially proctored and signed off by an attending physician.
Never dispose of unused fluid, put it in an extra pot and add to the cytology sample. Following cytology processing, the residual peritoneal fluid pellets were. Cytology of body fluid free download as powerpoint presentation. Specimen collection and processing instructions for.
Culture of the ascitic fluid was negative for bacteria and fungi. Many tumours retain the immunophenotype of the tissue from which they are derived and hence cytokeratin 7 and 20 immunostaining has potential value in diagnostic pathology. Paracentesis is a procedure in which a needle or catheter is inserted into the peritoneal cavity to obtain ascitic fluid for diagnostic or therapeutic purposes. If you continue browsing the site, you agree to the use of cookies on this website. Although fluid cytology poses diagnostic dilemma in few cases, still it is cost. Pdf ascitic fluid analysis in hepatocellular carcinoma. Cytology of body fluid pleural peritoneal pericardial ppt video. Specimen collection and processing instructions for medical laboratory test peritoneal fluid at geisinger medical laboratories. This is a fluid that is normally a part of an area of the body called the peritoneum, which consists of the digestive cavity. Ascitic fluid cytology numerous pleomorphic lymphoid cells in primary effusion lymphoma. In an attempt to identify and profile the population of ascites cells obtained from ovarian cancer patients, a novel method was developed to separate adherent ad and nonadherent nad cells in culture. If you continue browsing the site, you agree to the use of.
Ppt cytology of body fluid powerpoint presentation, free. Ppt ascites powerpoint presentation free to download id. If abdominocentesis is indicated, but is negative after a complete fourquadrant paracentesis has been performed, diagnostic. Pericardial fluid from the sac that surrounds the heart ascitic fluid, also called ascites or peritoneal fluid from the space in the belly scrape or brush cytology. Chapter 4 diagnostic pitfalls in effusion fluid cytology vinod b. Most ascitic fluid is transparent and tinged yellow.
586 1072 798 512 562 751 1434 945 1182 868 1347 408 535 1414 408 1135 1069 1338 95 516 255 437 1621 828 389 1568 1032 1304 1275 154 1439 210 551 743 978 1146 1403 1326 1117 485 160 422 177 387 711 541 655 529 228 551 13